ABSTRACT
In order to investigate the immune enhancement effects of Ophiopogon japonicus polysaccharide Ophiopogon japonicus (OJPS) on Newcastle disease (ND) live vaccine, chickens vaccinated against ND live vaccine was orally administered with the OJPS at high, medium and low concentrations respectively. In negative control group, chickens were given orally equal volume of physiological saline. On day 14, 21 and 28, the serum antibody titer, erythrocyte-C3b receptor rosette rate (E-C3bRR), erythrocyte-C3b immune complex rosette rate (E-ICRR) and peripheral lymphocyte proliferation were measured. The results showed that at most time points, the antibody titer, peripheral lymphocyte proliferation, E-C3bRR and elimination rate of immune complex of three OJPS administrating groups were significantly higher (P<0.05) than those in negative control group. It indicated that OJPS could significantly improve the immune efficacy of Newcastle disease live vaccine, Ophiopogon japonicus polysaccharide possessed synergistical immunoenhancement.(AU)
Subject(s)
Animals , Chickens/virology , Newcastle Disease/immunology , Ophiopogon/chemistry , Viral Vaccines/analysis , Adjuvants, Immunologic , Antibodies/blood , Erythrocytes/immunology , Lymphocytes/immunologyABSTRACT
Objective:To establish the determination method for total flavonoids in Cyperus rotundus and investigate the total fla-vonoids content in Cyperus rotundus from different habitats. Methods:Total flavonoids in Cyperus rotundus from different habitats were detected by ultraviolet spectrophotometry with rutin as the standard substance. Results:The absorbance of rutin had a good linear cor-relation with the concentration within the range of 0.064 3-0.642 6 mg·ml-1(r =0.999 1). The average recovery was 99.74%(RSD=2. 16%,n=6). The order of total flavonoids content in Cyperus rotundus from different habitats was Henan province >Sichuan province> Guangdong province >Anhui province > Halnan province >Shandong province. Conclusion:The method is easy and ac-curate, which can be applied in the quality control of Cyperus rotundus. The total flavonoids content in Cyperus rotundus from Henan province is the highest.